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proteosilver plus silver stain kit  (Millipore)


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    Millipore proteosilver plus silver stain kit
    Proteosilver Plus Silver Stain Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/proteosilver+plus+silver+stain+kit/proteosilver+silver+stain+kit/pm39082875-449-7-12
    Average 90 stars, based on 1 article reviews
    proteosilver plus silver stain kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Silver Staining:

    Article Title: Assembly of the Novel Five-Component Apicomplexan Multi-Aminoacyl-tRNA Synthetase Complex Is Driven by the Hybrid Scaffold Protein Tg-p43
    Article Snippet: Eluates from immunoprecipitation or SEC were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using pre-cast gradient gels (NuPAGE® Novex®4–12% Bis-Tris polyacrylamide gel, Life Technologies) with MES-SDS running buffer using the XCell SureLock® Mini-Cell as per the manufacturer's instructions. .. Proteins were then visualized by silver staining using ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich). .. For identification by mass-spectrometry (MS), proteins were first concentrated by precipitation with trichloracetic acid (1/6th volume), washed twice with ice-cold acetone, before separation by SDS-PAGE and staining with the Colloidal Blue Staining Kit from Life Technologies.

    Article Title: Host factor SMYD3 is recruited by Ebola virus nucleoprotein to facilitate viral mRNA transcription
    Article Snippet: .. The proteins were then visualized with ProteoSilver Plus Silver Stain Kit (Sigma Aldrich) according to the manufacturer’s instructions. ..

    Article Title: Optimizing ChIRP-MS for Comprehensive Profiling of RNA-Protein Interactions in Arabidopsis thaliana : A Telomerase RNA Case Study
    Article Snippet: .. SDS-PAGE gel was fixed in 50% ethanol with a 10% acetic acid solution overnight and stained with the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich) according to the manufacturer’s instructions. ..

    Article Title: Proteome analysis of vaccinia virus IHD-W-infected HEK 293 cells with 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS of on solid phase support N-terminally sulfonated peptides
    Article Snippet: IPG DryStrips and Pharmalyte pH 3-10 were obtained from GE Healthcare (Chalfont St Giles, Great Britain). .. Acrylamide, bis-acrylamide, iodoacetamide (IAA), thiourea, dithiothreitol (DTT), 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS), a-cyano-4-hydroxycinnamic acid (CHCA), acetonitrile (ACN), trifluoroacetic acid (TFA), 4-sulfophenyl isothiocyanate (SPITC), biammonium citrate, bovine serum albumin (BSA), sodium dodecyl sulfate (SDS), Trypsin Profile IDG Kit and the ProteoSilver Plus Silver Stain Kit were bought from Sigma-Aldrich (Taufkirchen, Germany). .. Tris (hydroxymethyl) aminomethane (TRIS) and solvents used (dichloromethane, ethanol, isopropanol) were supplied in analytical form by Merck (Darmstadt, Germany).

    Article Title: Mass Spectrometric Analysis of Ehrlichia chaffeensis Tandem Repeat Proteins Reveals Evidence of Phosphorylation and Absence of Glycosylation
    Article Snippet: After equilibration, the IPG strips were transferred to the top of a 4–12% Bis-Tris Zoom SDS-polyacrylamide gradient gel (Invitrogen) and overlaid with 0.5% agarose in Laemmli buffer for second-dimension SDS-polyacrylamide gel electrophoresis. .. Following electrophoresis, proteins were detected with ProteoSilver Plus Silver Stain Kit (Sigma), Zinc Stain (Biorad, Hercules, CA), or transferred to nitrocellulose membrane and detected with antibodies specific for TRP32, TRP47, TRP120 or E. chaffeensis as previously described , , . .. For recombinant TRP47, in-frame GST fusion proteins for full-length TRP47, amino (N)-terminal (TRP47 1–380 ) and carboxy (C)-terminal (TRP47 361–842 ) TRP47 were generated by PCR, amplifying the corresponding coding regions from E. chaffeensis Arkansas genomic DNA using custom synthesized oligonucleotide primers . pGEX-6P-1 plasmids encoding the GST-TRP47, GST-TRP47 1–380 (GST-NterTRP47), and GST-TRP47 361–842 (GST-CterTRP47) fusion proteins were transformed into BL21 strain of Escherichia coli (GE Healthcare Bio-Sciences Corp., Piscataway, NJ).

    Article Title: Proteome analysis of vaccinia virus IHD-W-infected HEK 293 cells with 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS of on solid phase support N-terminally sulfonated peptides
    Article Snippet: The size marker was RotiMark ® 10-150 kDa (Roth, Karlsruhe, Germany) and a Tris-glycine buffered system according to Laemmli (1970) was used [ ]. .. After electrophoresis, the proteins were silver stained with the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich) according to the manufacturer's instructions. .. Differences in the protein expression profile of the 2-D gels were identified by use of the image analysis software Melanie 7.0 (Swiss Institute for Bioinformatics).

    Article Title: Methods to incorporate machine learning analytics for optimizing protein purity, potency and quality in an on-demand production system for point-of-care delivery
    Article Snippet: Criterion TGX precast midi protein gel (4-20%) was used in the experiment following standard protocol with a Criterion electrophoresis cell, both obtained from Bio-Rad (cat. no. 1656001 and no. 5671093). .. For staining gels, ProteoSilver plus silver stain kit (Sigma-Aldrich, cat. no. PROTSIL2) was used. .. Known concentrations of cell-derived glucose binding protein (GBP), G-CSF (LifeTechnologies) or bovine serum albumin (BSA) were loaded along each sample and used as standard reference for determining the purity and the concentration of purified protein.

    Article Title: ORC1 enhances repressive epigenetic modifications on HIV-1 LTR to promote HIV-1 latency.
    Article Snippet: The human immunodeficiency virus type 1 (HIV-1) reservoir consists of latently infected cells which present a major obstacle to achieving a functional cure for HIV-1.. The formation and maintenance of HIV-1 latency have been extensively studied, and latency-reversing agents (LRAs) that can reactivate latent HIV-1 by targeting the involved host factors are developed; however, their clinical efficacies remain unsatisfac tory.. Therefore, it is imperative to identify novel targets for more potential candidates or better combinations for LRAs.

    SDS Page:

    Article Title: Optimizing ChIRP-MS for Comprehensive Profiling of RNA-Protein Interactions in Arabidopsis thaliana : A Telomerase RNA Case Study
    Article Snippet: .. SDS-PAGE gel was fixed in 50% ethanol with a 10% acetic acid solution overnight and stained with the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich) according to the manufacturer’s instructions. ..

    Staining:

    Article Title: Optimizing ChIRP-MS for Comprehensive Profiling of RNA-Protein Interactions in Arabidopsis thaliana : A Telomerase RNA Case Study
    Article Snippet: .. SDS-PAGE gel was fixed in 50% ethanol with a 10% acetic acid solution overnight and stained with the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich) according to the manufacturer’s instructions. ..

    Article Title: Mass Spectrometric Analysis of Ehrlichia chaffeensis Tandem Repeat Proteins Reveals Evidence of Phosphorylation and Absence of Glycosylation
    Article Snippet: After equilibration, the IPG strips were transferred to the top of a 4–12% Bis-Tris Zoom SDS-polyacrylamide gradient gel (Invitrogen) and overlaid with 0.5% agarose in Laemmli buffer for second-dimension SDS-polyacrylamide gel electrophoresis. .. Following electrophoresis, proteins were detected with ProteoSilver Plus Silver Stain Kit (Sigma), Zinc Stain (Biorad, Hercules, CA), or transferred to nitrocellulose membrane and detected with antibodies specific for TRP32, TRP47, TRP120 or E. chaffeensis as previously described , , . .. For recombinant TRP47, in-frame GST fusion proteins for full-length TRP47, amino (N)-terminal (TRP47 1–380 ) and carboxy (C)-terminal (TRP47 361–842 ) TRP47 were generated by PCR, amplifying the corresponding coding regions from E. chaffeensis Arkansas genomic DNA using custom synthesized oligonucleotide primers . pGEX-6P-1 plasmids encoding the GST-TRP47, GST-TRP47 1–380 (GST-NterTRP47), and GST-TRP47 361–842 (GST-CterTRP47) fusion proteins were transformed into BL21 strain of Escherichia coli (GE Healthcare Bio-Sciences Corp., Piscataway, NJ).

    Article Title: Proteome analysis of vaccinia virus IHD-W-infected HEK 293 cells with 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS of on solid phase support N-terminally sulfonated peptides
    Article Snippet: The size marker was RotiMark ® 10-150 kDa (Roth, Karlsruhe, Germany) and a Tris-glycine buffered system according to Laemmli (1970) was used [ ]. .. After electrophoresis, the proteins were silver stained with the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich) according to the manufacturer's instructions. .. Differences in the protein expression profile of the 2-D gels were identified by use of the image analysis software Melanie 7.0 (Swiss Institute for Bioinformatics).

    Article Title: Methods to incorporate machine learning analytics for optimizing protein purity, potency and quality in an on-demand production system for point-of-care delivery
    Article Snippet: Criterion TGX precast midi protein gel (4-20%) was used in the experiment following standard protocol with a Criterion electrophoresis cell, both obtained from Bio-Rad (cat. no. 1656001 and no. 5671093). .. For staining gels, ProteoSilver plus silver stain kit (Sigma-Aldrich, cat. no. PROTSIL2) was used. .. Known concentrations of cell-derived glucose binding protein (GBP), G-CSF (LifeTechnologies) or bovine serum albumin (BSA) were loaded along each sample and used as standard reference for determining the purity and the concentration of purified protein.

    Electrophoresis:

    Article Title: Mass Spectrometric Analysis of Ehrlichia chaffeensis Tandem Repeat Proteins Reveals Evidence of Phosphorylation and Absence of Glycosylation
    Article Snippet: After equilibration, the IPG strips were transferred to the top of a 4–12% Bis-Tris Zoom SDS-polyacrylamide gradient gel (Invitrogen) and overlaid with 0.5% agarose in Laemmli buffer for second-dimension SDS-polyacrylamide gel electrophoresis. .. Following electrophoresis, proteins were detected with ProteoSilver Plus Silver Stain Kit (Sigma), Zinc Stain (Biorad, Hercules, CA), or transferred to nitrocellulose membrane and detected with antibodies specific for TRP32, TRP47, TRP120 or E. chaffeensis as previously described , , . .. For recombinant TRP47, in-frame GST fusion proteins for full-length TRP47, amino (N)-terminal (TRP47 1–380 ) and carboxy (C)-terminal (TRP47 361–842 ) TRP47 were generated by PCR, amplifying the corresponding coding regions from E. chaffeensis Arkansas genomic DNA using custom synthesized oligonucleotide primers . pGEX-6P-1 plasmids encoding the GST-TRP47, GST-TRP47 1–380 (GST-NterTRP47), and GST-TRP47 361–842 (GST-CterTRP47) fusion proteins were transformed into BL21 strain of Escherichia coli (GE Healthcare Bio-Sciences Corp., Piscataway, NJ).

    Article Title: Proteome analysis of vaccinia virus IHD-W-infected HEK 293 cells with 2-dimensional gel electrophoresis and MALDI-PSD-TOF MS of on solid phase support N-terminally sulfonated peptides
    Article Snippet: The size marker was RotiMark ® 10-150 kDa (Roth, Karlsruhe, Germany) and a Tris-glycine buffered system according to Laemmli (1970) was used [ ]. .. After electrophoresis, the proteins were silver stained with the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich) according to the manufacturer's instructions. .. Differences in the protein expression profile of the 2-D gels were identified by use of the image analysis software Melanie 7.0 (Swiss Institute for Bioinformatics).

    Membrane:

    Article Title: Mass Spectrometric Analysis of Ehrlichia chaffeensis Tandem Repeat Proteins Reveals Evidence of Phosphorylation and Absence of Glycosylation
    Article Snippet: After equilibration, the IPG strips were transferred to the top of a 4–12% Bis-Tris Zoom SDS-polyacrylamide gradient gel (Invitrogen) and overlaid with 0.5% agarose in Laemmli buffer for second-dimension SDS-polyacrylamide gel electrophoresis. .. Following electrophoresis, proteins were detected with ProteoSilver Plus Silver Stain Kit (Sigma), Zinc Stain (Biorad, Hercules, CA), or transferred to nitrocellulose membrane and detected with antibodies specific for TRP32, TRP47, TRP120 or E. chaffeensis as previously described , , . .. For recombinant TRP47, in-frame GST fusion proteins for full-length TRP47, amino (N)-terminal (TRP47 1–380 ) and carboxy (C)-terminal (TRP47 361–842 ) TRP47 were generated by PCR, amplifying the corresponding coding regions from E. chaffeensis Arkansas genomic DNA using custom synthesized oligonucleotide primers . pGEX-6P-1 plasmids encoding the GST-TRP47, GST-TRP47 1–380 (GST-NterTRP47), and GST-TRP47 361–842 (GST-CterTRP47) fusion proteins were transformed into BL21 strain of Escherichia coli (GE Healthcare Bio-Sciences Corp., Piscataway, NJ).



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